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Testing antibotics on bacteriaTo determine the effect of ten different antibiotics on two different types of bacteria I will test six antibiotics on Escherichia coli and sixantibiotics on Bacillus subtilis On Escherichia coli I will test tetracycline chloramphenicol furadantin nalidixic acid triple sulfa and kanamycin On Bacillus subtilis I will test streptomycin erythromycin novobiocin tetracycline chloramphenicol and penicillin As a side observation I would also like to see if Bacillus subtillis shows resistance to penicillin The use of penicillin is being reduced because of the resistance many types of bacteria are developing against it HypothesisMy hypothesis is that penicillin will inhibit the most growth against Bacillus subtilis and that tetracycline will stop Escherichia coli more effectively than the othersRationaleI feel that this experiment is valid because it shows how different antibiotics react to different types of bacteria It also points out the fact that not all antibiotics work the same or that they work at all on all types of bacteria Materials1 bottle of tryptic soy agar1 culture of Escherichia coli1 culture of Bacillus subtilis4 sterile petri dishes1 pack of test discs2 sterile pipettes2 tubes of physiological saline1 forceps1 wax pencil2 sterile swabsMethod1 Loosen the cap of the tryptic soy agar to allow it to vent Place the bottle in a water bath at 100 degrees Centigrade so that the water level reached the level of the medium The agar will melt in about 20 minutes 2 Gradually cool the medium to about 45 degrees Centigrade by letting the water bath cool Then let the medium sit for 10 minutes 3 Pour two plates for each species of bacteria The agar should be approximately 5 mm deep Cover each petri dish immediately after poring to prevent contamination 4 After pouring the plates allow the agar a few minutes to solidify You can check this by tilting the plate If the agar flows to one side more cooling time is needed 5 Add a tube of
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