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Chromatography Research Most likely the average person off the street couldnt tell you exactly what chromatography is I however can tell you this and much more relating to the subject Chromatography is defined by Websters Dictionary as a process in which a chemical mixture carried by a liquid or gas is separated into components as a result of differential distribution of the solutes as they flow around or over a stationary liquid or solid phase Not all mixtures can be separated using any one form of chromatography so various methods have been developed over the years The most commonly used forms of chromatography are column high performance liquid ion paper thin layer and gas chromatography By separating the individual components of different mixtures scientists are able to study the components much easier and more efficiently In 1906 Russian botanist Mikhail Tswett became the discoverer of chromatography when he poured a petroleum-ether extract of green leaves over a column of powdered calcium carbonate in a vertical glass tube By doing this he was able to separate each of the pigments and thus study each in depth It wasnt until the 1930s that chromatography became widely used in the scientific community but as its popularity increased discoveries abounded Column chromatography is one of the more common forms of chromatography because it can be used for very delicate separations that are hard to achieve using other methods For this method a vertical glass or plastic tube usually 50 cm tall and 1 cm wide is packed with a stationary phase such as aluminum oxide or calcium carbonate Then the mixture to be separated is dissolved in a solvent and added to the top of the column where it the mobile phase will then work its way down and separate into its different components In high performance liquid chromatography the particle size of the stationary phase is greatly reduced in order to increase surface area and thus
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