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Pineapple Lab Introduction-For some strange reason gelatin and fresh pineapple do not get along When juice of the fresh pineapple unchanged from its natural form comes into contact with the gelatin it changes the gelatin from a solid into a liquid that cannot be reformed into gelatin A very interesting occurrence in life and we want to why this is We have a hypothesis and it is that the juice from the pineapple contains enzymes which break down protein So to prove this we have to do multiple experiments that test the qualities of enzymes so it can be narrowed down to be one thing which we believe is enzymes All our experiments have to be controlled experiments or experiments that only have one variable factor and a control group to compare the results to We will use Petri dishes or sterile circular plates where I can observe the experiments as they take place 1 Change of Temperature Hypothesis- The colder pineapple juice will break down the gelatin slower than the room temperature pineapple juice Also the warmer pineapple juice will break down the gelatin faster than the room temperature pineapple juice I think this because when molecules heat up they vibrate faster and therefor the enzyme will touch more substrates which will get more gelatin broken down However the boiled pineapple juice will not work because the enzyme will be denatured because of the heat Procedure- Take four even amounts of pineapple juice and put it in different test tubes Put one test tube in a tube full of ice water another in hot water and leave them in for fifteen minutes Also put one test tube over a flame until it boils for a short amount of time and leave one at room temperature Put the juice from each test tube onto different piece of gelatin after waiting for the boiled pineapple juice to cool so it wont melt the gelatin and wait
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