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Study Of The Enviroment On The Cell Membrane Dave Lima SBI OA1 Mr Swanson 10499 Purpose To study the effect of environmental changes in the permeability of living beet root cells Procedure A The effect of pH on the cell membrane Materials Method 1 Cut a beet root into slices approximately 2mm thick Use a cork bore to cut out thirty-five 35 slices 2 Place the discs in a 250 mL beaker and rinse thoroughly in cool running tap water The red pigment collected comes from the damaged cells and can be used to analyze the chemical nature of the pigment 3 The pigment in a beet anthocynanin should be analyzed before beginning This can be done simply by first adding a few drops if concentrated acid to a sample if beet juice 4 The resulting solution can be compared to a second sample made by adding a few drops if concentrates base to another sample of beet juice Note the colours of each Given stock solutions of 01 molL HCl and 01 molL NaOH and graduated cylinders prepare the following solutions in separate test tubes a 10 mL of 01 molL HCl b 10 mL of 001 molL HCl c 10 mL of 0001 molL HCl d 10 mL of distilled water e 10 mL of 0001 molL NaOH f 10 mL of 001 molL NaOH g 10 mL of 01 molL of NaOH 5 Place five 5 beet root discs in each of the 7 test tubes 6 Leave them for 15 minutes and then record your observations Agitate gently and continue to observe them at 15-minute intervals until no further changes occur Procedure B The effects Of An Organic Solvent Materials Methods 1 Prepare beet root discs as for Procedure A The number required will depend on the control you design 2 Place
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