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Word Count: 308
The enzyme amylase will catalyze the hydrolysis of starch to maltose when the pH is near 70 But when the HCl is added to the solution the amylase will be denatured which results in the enzyme being deactivated The iodine serves as an indicator for the presence of starch Iodine I2 will reach with iodide ion to produce the I3- ion This ion will form a dark blue complex with the starch molecule Like most chemical reactions the rate of an enzyme-catalyzed reaction increases as the temperature is raised A ten degree Centigrade rise in temperature will increase the activity of most enzymes by 50 to 100 Variations in reaction temperature as small as 1 or 2 degrees may introduce changes of 10 to 20 in the results In the case of enzymatic reactions this is complicated by the fact that many enzymes are adversely affected by high temperatures As shown in Figure 13 the reaction rate increases with temperature to a maximum level then abruptly declines with further increase of temperature Because most animal enzymes rapidly become denatured at temperatures above 40C most enzyme determinations are carried out somewhat below that temperature Over a period of time enzymes will be deactivated at even moderate temperatures Storage of enzymes at 5C or below is generally the most suitable Some enzymes lose their activity when frozen As amylase breaks down starch less and less starch will be present and the color of the solution if iodine is added will become lighter and lighter Enzymes are biological molecules that catalyze many different chemical reactions With few exceptions all enzymes are proteins and each enzyme is specific to a certain chemical reaction Enzymes must maintain a specific three dimensional structure in order to function properly If an enzymes structure is altered by heat or harsh chemicals it may not function at all This breakdown denaturation of an enzymes
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