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PROCEDURE Between January and March 1996 sexually mature winter flounder were captured from Shark River NJ by Steve Teluvech of Richard Stockton College Nacote Creek Marine Science Research Center The flounder were then kept in an aerated tank with a maintained temperature between 1-10C and a salinity between 114 - thirty-three ppt in the said research center Each individual flounder was injected with a prepared carp pituitary hormone extract GtH according to their body weights to induce spawning Using a 20 microliter syringe the extract was injected into the intraperitoneal cavity Two injections within four hours per specimen were given per day After the appearance of a swollen abdomen the injections were terminated The flounder were allowed time for ovulation before the milt semen and the eggs were stripped and collected into a container The eggs and the milt were allowed to mix for five minutes to permit optimal fertilization Then the egg mixture was washed with clean sea water with a 20-30 ppt salinity and 5-10C temperature to remove excess organic matter The mixture was bathed in a 1 bentonite solution rinsed and then equally divided and placed into aerated 2 liter plastic containers To maintain the temperature the containers were then placed in the same tank with the flounders Aeration was controlled to maintain adequate dissolved O2 level A certain volume if fungicide was added to prevent fungal growth Observations of the development of the eggs were taken daily under dissecting microscopes Unfertilized eggs were collected and discarded A sample of eggs in different stages of development was also collected and preserved in 10 formalin Twenty percent of the sea water of the containers was also a changed daily and transferred into another container INTRODUCTION Winter flounder Psuedopleuronectus Americanus is a benthic marine fish that occurs from the Gulf of St Lawrence to the Chesapeake Bay They do not spawn well in laboratories or aqua cultured systems
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