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Analyzing the origin of adaptive mutations in Saccharomyces cerevisiae Abstract Biological mutations play an integral role in the long-term survival of populations due to the potential benefit that the genetic variance provides The main objective in this laboratory was to utilize the replica-plating technique to determine whether copper-tolerant mutants in Saccharomyces cervisiae arise spontaneously or if they are induced by their environment This type of bakers yeast is ideal for mutation experiments due to the specimens quick reproduction ability Following several serial dilutions individual yeast colonies were able to be produced on a agar medium Through the technique of replica-plating cells from these colonies were able to be transferred to three separate petri plates in the same spatial arrangement they were found on the original plate One of these plates was prepared with a standard agar medium while the other two contained a copper-based agar The cultures were consequently refrigerated in order to prevent growth after the yeast reached 3 mm in diameter The results of the experiment showed that the location of the mutant colonies were generally identical on all three petri plates These findings suggest that mutations are pre-existing and occur spontaneously due to the fact that mutants of this nature would be found in positions identical with those on the first plate Introduction The main objective in this laboratory was to utilize the replica-plating technique to determine whether copper-tolerant mutants in Saccharomyces cervisiae arise spontaneously or if the environment induces these mutations This experiment is very significant because it exhibits the fact that mutations occur spontaneously at a characteristic rate The central focus in this laboratory was to explore the time that adaptive mutants arise in a population If a mutation is pre-existing and occurs spontaneously it is possible for the characteristic to be favored in an event that the environment changes Lab Manual p 27 On the other hand induced mutations occur only
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