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Concentration of Food Colors in Artificially Colored BeveragesFake Hall rm405 SUNY Cobleskill NYThis experiment was performed to investigate and determine the concentration of food coloring in Food Club Brand Cranberry-Ginger Ale Methods used in the experiment include comparing the color of the sample to the 3 controls at 5ppm 10ppm and 15ppm by an approximate assessment with just simple eyesight and the use of a spectrophotometer The color of the sample of Food Club Ginger Ale seemed to have a darker color than that of the three controls well above 15 parts per million By using the spectrophotometer set on the absorption calibration we tested the sample at wavelengths lambda of light between 400nm and 780nm These tests were performed to determine the colors preeminently absorbed by the sample In order to properly measure the sample with the spectrophotometer we first had to dilute the sample to a color intensity lighter than 15 ppm We did this by pouring 25 ml of the sample into a 50 ml graduated cylinder and then diluting it with distilled water to the 50 ml line This created a dilution factor of 5025 or 2 To use the spectrophotometer we had to obtain two cuvettes One cuvette was filled 23 full with distilled water and the other cuvette filled 23 full with our diluted fruit drink sample We first set the wavelength of the spectrophotometer at 400nm We then had to zero the instrument by placing the cuvette of distilled water into the machine and pressing the 100 T0A button After the instrument was zeroed we placed the fruit drink cuvette into the instrument and recorded the absorbance We repeated these steps but increased the wavelength by 20nm each time It was also necessary to re-zero the spectrophotometer at every increase in wavelength by using the method stated above We found that Food Club Ginger Ale strongly absorbs light between the wavelengths 480nm and 540nm and transmits colors
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