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AbstractBy using bacterial conjugation method to perform an experiment that positively influences the genetic makeup of bacteria Escherichia coli the donor was mixed to the recipient then the mixture incubated for 30 minutes After that transferred the mixture into two sterile water blanks and removed them into two agar plates one contained streptomycin and thiamine STRTHI 110 and the other was STRTHI 1100 One may assume that these bacteria would grow due to the transferring genetic materials between the donors to the recipient The second half of the experiment was compared the effect of both donor and recipient ability to grow on a specific minimal medium set up control variables which pipetted each donor and recipient into each STRTHI plates and minimal medium supplements with amino acids MPLTT plates The donor and recipient samples expect no grow on STRTHI but grow on MPLTT This suggests that in order to survive bacterial culture require a specific amount of nutrients that present in the medium to help them reproduceIntroductionUnderstanding the bacterial conjugation has enabled development of genetic engineering For instance scientists often use bacterial conjugation to make useful substances for human Lab manual 60 This is a valuable concept and simply referred to Joshua Lederberg and Edward L Tatum experiment with bacteria Escherichia coli Their proof was showing that bacteria can actually exchange genes when they reproduce which very similar to animals and plants They tested two types of double mutant bacteria each lacking the ability to make different nutrients but can making the other one required They placed the two types together on the agar plate without any nutrients However when the two strains were grown together they found there were numerous bacterial colonies on the plate They concluded that there must be an exchange genetic material between the two cells and later called this phenomenon conjugationIn this experiment a gram negative bacterium Escherichia coli or E coli was used because it does not harmful to
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