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Enzyme Activity Abstract The purpose of this experiment is to test the effects of different sugar types with pH and temperatures We are testing to see if there is a difference in reaction and reaction time when placed into different temperature and pH We are looking for changes in color solidity reaction time etc My hypothesis is that at different temperatures and amounts of pH there will we different reactions Introduction The overview of these experiments is to test enzymes and see what kinds of reactions they have in different setting Temperature pH sugars etc are all part of this testing process The importance of this experiment is to gain better knowledge of enzymes so that we can know more about them Enzymes are very important in everyday life because they keep people from getting diseases and they help us to do simple thing like digest foods Procedure Collect lab supplies and begin testing First we mix 2ml of each sugar with 2ml of Benedicts Solution We then placed these tubes in hot water and after three minutes noted a color change The point of this test was to find out which were single sugars and which were double sugars After everything we record our findings For our next test we take the same amounts of sugar and Benedicts solution but we increase the enzyme to see if the amount of the reaction will increase We mix the correct solution of sulfur sucrose solution enzyme stock solution and water and let sit at room temperature for fifteen minutes We then record our findings Our next test will be the effects of temperature on enzyme activity We place buffer enzyme and sucrose solutions into four different temperature settings We then mix them after six minutes We let them sit for another fifteen minutes and record our findings Next we test the effects of pH on enzymes We first add
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