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INTRODUCTION The enzyme catalase speeds up the decomposition of Hydrogen Peroxide into water and oxygen as shown here 2H2O2-------------------2H2OO2 It is one of the fastest known enzymes and its turnover number is 6 million which means the number of substrate molecules which one molecule of the enzyme turns to products per minute This can be demonstrated by putting a piece of liver into a beaker of Hydrogen Peroxide the fizzing shows a demonstration of the enzyme in action AIM My aim is to examine how the concentration of the substrate hydrogen peroxide affects the enzyme catalase INVESTIGATION I am going to investigate the effect of varying the substrate concentration on enzyme catalase I am going to use 8 different concentrations and record the time taken to collect 20ml of gas in the gas syringe I will repeat all the 8 concentrations twice so I can see if they match spot out any anonymous results and also I can work out the average time it takes to produce 20ml of gas at the certain concentrations I will vary the concentrations by increasing and decreasing the amounts of Hydrogen Peroxide and water PLAN First of all I will ensure I have enough enzyme solution for the whole experiments so the enzyme solution is standardised With the results I get I will try to work out the Vmax I will do this experiment at room temperature so the enzymes get enough kinetic energy to collide I will need 80ml of the enzyme solution because I will use 5ml for all of the experiment and I will do 8 different concentrations and I will repeat this concentrations twice so that is 5x8x2 80 First of all I will set out the equipment as I will show in the diagram then I will cut some pieces of liver which is the source of the enzyme Then I will grind the pieces
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