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Introduction to PCR lab In this experiment polymerase chain reaction PCR is used to amplify a short piece of DNA from the human chromosome 8 A short insertion of a DNA sequence in this case Alu which is found to be within the tissue plasminogen activator gene on chromosome 8 is observed very carefully Even though the DNA from human to human does not differ a whole lot and has many similarities the diversity among regions of the human chromosomes is high Because of the fact there is a variety a sequences of TPA-25 it is said to be polymorphic meaning many forms are possible This polymorphism helps us define the basis for diagnosis of genetic deseases forensic identification and paternity testing An Alu is a short interspersed repetition of DNA elements that are scattered throughout the primate genome The Alu in this element had a DNA sequence of 400bp which means if you insert the mixture of PCR and DNA and have the TPA25 present into Agarose gel Electrophoresis a fragment line will form at 400bp It is called Alu after the single recognition site for the restriction enzyme AluI which is detected in the middle of the Alu element The Alu element called TPA-25 that is used in this experiment is located within an intron if the tissue plasminogen activator gene Because the insertion of it is only present in some individuals and in other not we can get three main result The individual can either be homozygous for the trait heterozygous meaning one allele has it and the other one doesnt or homozygous but does not show the trait in either allele In this experiment PCR will do the job to detect whether a person is positive for the TPA-25 insertion PCR is a process in which a particular DNA segment from a mixture of DNA chains is rapidly
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