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Finding out about photosynthesis Date Partners Aim To be able to explain the conditions necessary for photosynthesis and to compare experimental results to a control To recognise tests for some inorganic substances found in plants Materials h A plant with variegated leaves in a pot which has been in the dark for 24 hours prior to the class h Iodine h Alcohol h Potassium hydroxide h Hotplate or Bunsen burner with tripod h Petri dish h Funnel h 2 flasks 500ml h 2 beakers h Aluminium foil h Forceps h Retort stands and clamps h Cottonwool h Grolux light or other light source Method Day 1 The plant you will use has been left in the dark for 24 hours prior to the class 1 Take one leaf of the plant and test it for the presence of starch as follows a Place the leaf in a small beaker of alcohol b Place the beaker in a water bath c Bring the water in the water bath to the boil d Leave until the alcohol in the beaker containing the leaf boils e Using forceps remove the leaf carefully form the alcohol f Wash it in the boiling water to soften it g Spread the leaf on a petri dish h Pour iodine over the whole leaf i Iodine changes to a blue-black colour in the presence of starch Note whether this occurs Part A Setting up your experiment You will investigate the three conditions necessary for photosynthesis h Chlorophyll h Light h Carbon dioxide You can use different leaves on one plant to test all three conditions Use figure 25A as a guide to setting up the experiment Is chlorophyll necessary for photosynthesis 2 a Select a leaf but leave it attached to the plant Sketch it showing the arrangement of the green and non-green areas b Record all
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