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Introduction Osmosis is the diffusion of water across a selectively permeable membrane in living organisms from a hypotonic to a hypertonic solution Raven Johnson 2000 The speed of which one substance diffuses from one area to another is based upon the free-energy gradient between the two areas For example if two substances differ greatly between their areas it allows the gradient between them to be steep which results in rapid diffusion If however the gradient between the two substances is equal there is no diffusion and the net movement stops Osmosis follows the same laws as those of diffusion but it will always refer to water as the principal solvent in cells Because water is the universal solvent in cells its diffusion across selectively permeable membranes is critical to living organisms Gibson-Brown Lab Notes 2000 Osmosis can be simulated by using dialysis tubing to form bags to represent cells under different conditions and measuring the direction and rate of osmosis The following experiment is to use the bags to measure the rates of osmosis in each bag Each bag in the experiment is a model of a cell The experiment requires the usage of four bags labeled A B C and D The bags will all be filled with a pre-measured solution for each of them and set into containers containing the same solution at a different concentration level In bag A 1 Sucrose B 1 Sucrose C 25 Sucrose and D 50 Sucrose Bag A will be placed into a separate container than that of the other three bags It is now where the procedure begins HYPOTHESIS If the higher gradient between two areas results in a faster osmosis effect then the bag or bas that will have the fastest rate of osmosis will be Bag A and Bag D PROCEDURE To begin the experiment obtain eight clamps and four pieces of water soaked dialysis tubing 15
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