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AbstractIn this study treatment of palm oil mill effluent POME was carried out under anaerobic fermentation process with the intention to produce bio-hydrogen by micro flora Experiment was investigated in 500mL bioreactor under mesophilic operation at and different pH value Raw POME was collected from cooling pond which is final discharge of effluent from the mill and POME sludge was collected from the anaerobic pond of a POME treatment plant at same Labu palm oil mill respectively Source of inoculum was used is POME sludge as hydrogen producing bacteria A batch reactor was found producing higher hydrogen at optimum parameter of pH 55 and 10 POME sludge wv with maximum hydrogen production yield of 598896 mL H2 L-med and maximum hydrogen percentage in the biogas of 36 were obtained at pH 55 Throughout the study there is no methane gas was observed in the evolved gas mixtureKeywords Bio-hydrogen POME sludge Raw POME Microflora1 IntroductionCurrently in Malaysia there are 432 palm oil mills included in Sabah and Sarawak As mentioned by Rupani et al 2010 it has been estimated that 5- 75 tonnes of water is required for the production of 1 tonne crude palm oil CPO and more than 50 of the water ends up as palm oil mill effluent POME Pre-discharge POME treatment is inadequately performed by palm oil industriesand this is mostly due to the conventional treatment technologies which are predominantly time consuming environmentally not sustainable and require high cost of operation In addition based on the characteristics of treated POME remain biochemically incompatible for discharge and still give pollution into environment which is the continuous discharge of untreated or low-level-treated POME into the environment has culminated into high rates of diminishing freshwater reserves and contaminated marine and land systems Due to the contents of high total solids in POME attempt have been made to reuse and recycle the palm oil mill effluent POME by convert this waste into valuable products such
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