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Eicosapentaenoic acid EPA C205 3 and Docosahexaenoic acid DHA C226 3 are important 3 polyunsaturated fatty acids PUFAs while Arachidonic acid AA C204 6 is a vital 6-PUFA They have numerous neutraceutical and pharmaceutical applications Shahidi and Wanasundara 1998 Horrocks and Yeo 1999 EPA and DHA are important in the treatment of Atherosclerosis Cancer Rheumatoid Arthritis Psoriasis and diseases of old age such as Alzheimers and age-related muscular degeneration Drevon et al 1993 Simopoulos1999 AA and DHA are of special importance in the brain and blood vessels and are considered being essential for pre and post-natal brain and retina development Crawford 2000 The eicosanoids such as prostaglandins prostacyclins and leukotrienes derived from 3-PUFAs are important for infant growth modulatory vascular resistance and wound healing Simopoulos 1999 Nettleton 1995 Illman et al 2000 Zhila et al 2005a and b suggested that nitrogen deprivation can improve the lipid content in microalgae Concentration of nitrogenous compounds in the culture media can regulate intracellular lipidtriglyceride accumulations Gordillo et al 1998 Limonet et al 2007 Pruvost et al 2009 Roessler 1990In general it appears that microalgae maintain lipid synthesis under nutrient starvation or stress condition when the productionfunctioning of other types of cellular constituents are curtailed Hu 2004 Miao and Wu 2006 stated that heterotrophic cultures made it possible to reach higher lipid content rather than photoautotrophic cultures The current recommendation of the US Institute of Medicine for Alpha-linolenic acid ALA intake is 16 g d-1 for men and 11 g d-1 for women 10 of which can be consumed as EPA plus DHA Kris-Etherton et al 2009This study aimed to elucidate the effect of nitrate and carbohydrates on lipid and polyunsaturated fatty acid methyl ester fatty acid PUFAMEFA production from the unicellular green microalgae Tgracilis and Pconvolutae The possibility for effective PUFAMEFA production in photoheterotrophic culture system was discussedTwo green unicellular marine microalgae species T gracilis and P convolutea used in this study strains were procured from Central
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