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Instruction Manual The purification process Recrystallization Filtration Jason Williams 09-13-00 NATS 4310 Table of Contents Preface Chapter 1 Equipment involved and preparation Glassware and accessories Proper sterilization of equipment Safety precautions Chapter 2 Setup procedure before purification Equipment setup Choosing of solvent Chapter 3 Gathering of an unknown compound and the first step filtration Collection Filtration Repeat Filtration and Cleaning Collection and measuring of crude sample Chapter 4 Recrystallization process Solvent addition Induction of Crystals Collection Chapter 5 Evaluation methods for purity Small scale test More accurate tests Chapter 6 Frequently asked Questions and trouble shooting Index Preface To begin the intent of this booklet is to give instructional advice The instructions given will provide a basis to allow an individual to perform the necessary task to undergo the purification process The design of the manual is to instruct students in a college laboratory setting In particular the area of science that the manual will focus on is Organic Chemistry Students will be able to refer to this instruction guide throughout the semester because this process will be put in use often The purification process if an essential part of chemistry and the student must recognize its importance Without a purified product many of the drugs on the market would not be on sale Even though these purification procedures are on a small scale it will provide a starting point for those who are just beginning Organic Chemistry The skills needed to purify a product will come with practice and help from the instructor but this manual will give the initial directions to help the student on purification Once mastered more techniques will help the student to gain a product that is pure enough to put on the market Chapter 1 The Equipment involved and preparation The first step in understanding any laboratory process is understanding the equipment needed Also there is a need for the
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