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Word Count: 636
The purpose of this experiment was to measure absorbance of enzymes activity at different times The experiments simplified by using substrate as a phenolphthalein monophosphate PMP Shown in fig1 Phenolpthalein was pH indicator it appeared colourless below pH 9 then turned pink above pH 10 In this experiment alkaline phosphatase used as an enzyme which produces the hydrolysis of ester linkage 103 BMS Enzyme Kinetics Laboratories 1 and 2 schedule Enzymes are protein catalysts A catalyst means substance that raises the rate of reaction without being modified Enzymes are proteins that placed into complex shapes and picks up smaller molecules in it In general the small molecules are called substrate and this procedure is called active site The factors that affected the rate of reaction and cause the enzyme has been denatured extreme temperature or great pH Prescotts Principles of Microbiology Chapter 9 Enzyme kinetics was the method that catalyzed the enzyme reactions calculated by making kinetic measurements on reaction These calculations measured catalyzed enzyme reaction at different concentration rates of substrate and enzyme The catalyzed enzyme are plotted on graph V known as reaction velocity against concentration of substrate S All of these terms meet in a common term it is called Michaelis-Menten theory and it is based on substrate concentration This equation is all about relationship between the reaction velocity V and the substrate concentration S and two measurements which firm in the equation Vmax and Km These two symbols that used in equation is refer to the enzyme penetrated with substrate at maximum velocity Vmax and Michaelis-Menten constant Km Shown in fig 2 The Km value is more important in this equation because it is represented the enzyme affinity of substrate and substrate proposed for the enzyme reach to the half maximal velocity used as similar affinity of an enzyme for its substrate Low Km value means reduced the substrate concentration at which an enzyme
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