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Word Count: 308
ProcedureThe procedure consisted of extracting an unknown soil sample Sc preparing calibrated solutions and measuring the absorbance values of the soil sample calibrated solutions and prepared solutionsData to evaluate the absorbance value of the unknown soil sample Sc were obtained by using the following procedures Approximately 5g of the sample was weighed out and then transferred to a 125 mL Erlenmeyer flask where 50 mL of extraction fluid was added in conjunction with the soil sample The mixture was stirred for 45 minutes by using Teflon coated stir bar placed in the Erlenmeyer flask After being stirred the fluid mixture was filtered in a Buchner funnel until the filtrate was clear in color 5 ml of the filtrated was transferred to a 50 mL volumetric flask and then diluted to the mark on the flask the final solution was inverted 13 times to properly mix the solutionTo evaluate the absorbance value of the prepared concentration solutions of 1 part per million ppm and 2 ppm respectively were obtained by using the following procedures 100 mL of the 1000 ppm Manganese Mn stock was pipetted into 100 mL and 50 mL volumetric flasks respectively The solutions were diluted with deionized water filled to the mark on the flasks Both flasks were inverted 13 times to properly mix the calibrated solutionsThe atomic absorption spectroscope was calibrated by the lab instructor The following data was obtained by measuring the standard solutions 05 ppm Mn produced a measured absorbance value of 0040 1ppm had a measured value of 0085 2ppm had a measured value of 0166 and 4 ppm had a measured absorbance value of 0330 The following data was obtained by measuring the prepared solutions 20 ppm Mn produced a measured absorbance value of 0185 and the 10 ppm Mn produced an absorbance value of 0094 The unknown soil sample Sc produced a measured absorbance value of 0056
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