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SPECTROPHOTOMETER ANALYSIS of ENZYMATIC SOLUTIONS Abstract This experiment involved the use of a spectrophotometer and solution mixed by us in the laboratory The spectrophotometer was used to analyze the speed of the reaction taking place in the sample through the use of light A solution called guiacol was used because its reactivity with oxygen causes it to turn a brown color adding pigmentation to the solution Jacklett 2000 All the solutions were made using guiacol turnip extract H202 and dH20 The graphs show how the varying amounts of substrate and enzyme concentration affected the percentage of light absorbed by the solution in the spectrophotometer Introduction This experiment was conducted in order to ascertain the effect of enzyme and substrate concentration on enzymatic reactions taking place in solutions we prepared An enzyme is a polypeptide macromolecule that catalyzes biochemical reactions Bugg 1999 A spectrophotometer was used in order to detect the rate of light absorption by the solution This number acted as a speedometer for the reaction The quicker the solution got darker the faster the reaction was going When the spectrophotometer began giving off higher numbers this indicated that the reaction was near its end because the solution was at one of its darkest points All the solutions were made using mixtures of guiacol turnip extract H202 and dH20 in test tubes Peroxidase is the enzyme we worked with in this laboratory It is responsible for catalyzing the breakdown of hydrogen peroxide water and oxygen Peroxidase is also responsible for the removal of toxic by products of hydrogen peroxide from the cell to prevent damage By this experiment we hoped to support the hypothesis that if the amount of the substrate or enzyme increases the rate of absorption and reaction increases
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