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Effects Of Solutions On Ion Transport Across Membranes Abstract Concentration gradients exist between two different salt molarity solutions on opposite sides of a membrane Movement of ions across these sides can be achieved by either active or passive diffusion In our first three experiments we used these concentration gradients to determine the characteristics of selective permeability of an artificial membrane to potassium chloride KCl the potential difference across the skin of a frog Rana pipens and the effect of inhibitors in the same frog membrane epithelium We measured the potential difference between the two sides of the artificial membrane with a voltmeter These measurements were then compared with the theoretical numbers measured using the Nernst equation The measured results were extremely close to the values measured by the Nernst equation but even more accurate compared to the values from the Goldman equation These results showed that as the difference in concentrations increased the potential across the membrane increased steadily Using the membrane of frogs skin the potential difference across the membrane was also measured as well as the current with a voltmeter and a short circuit current SCC These measurements showed that as the sodium concentration was decreased both the potential and the current decreased as well In addition another experiment was conducted in order to test the effects of inhibitors and hormones on the transport through the frog membrane Results showed that when ouabain and cyanide were added to the epithelium potential decreased steadily but the addition of vassopressin increased potential due to the increase of sodium transport across the membrane Because cyanide is an ATP inhibitor and ouabain is a physical barrier of the NaK pump this experiment concluded that active transport in the frog skin is indeed an active process and is not possible without ATP Introduction There exists a separation of electric charge across plasma membranes known as a membrane
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