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AbstractThe prey proteins interacted with Lin 42-D in C elegans This is proved by growth tests and x-gal assays that confirmed those results This experiment was to classify unknown genes which were associated with regulation of the developmental timing in C elegans The experimental result need to be further confirmed through other experimental methodsIntroductionIt is revolutionary that protein interactions could be detected in the early 90s Due to this the yeast hybrid system was developed and improved gradually Fields 1989 Scientists are figuring out that protein interaction is complex process Protein-protein interaction which composed this complex process is important in every cellular process and it is the main point to understand the biological system Variations of theyeast two-hybridwere developed to conduct screens in the presence of a co-factor or an enzyme required for a given post-translational modification of the protein partnersNaba 2008 Finally yeast hybrid screening experiment contrived for small molecule-protein interactions Licitra 1996 RNA-protein Putz 1996 andDNA-protein Li 1993How does the very small cell develop into a mature human being To grow or developdevelopmental timing is important issue Development such as the evolution oftissues the development of organs or the rotation of cell divisions needs to have the righttiming Caenorhabditiselegans has molecules which elements of developmental timingin heterochronic genes This heterochronic pathway in C elegans may have preservedmechanisms and the mechanisms have ascended to resolve the problem of animaldevelopmental timing Moss 2007 There is another reason developmental timing is importantin human For the specific animal life the regulation of developmental timing processshould be properly timed OConnor M ndThe Lin-42 period controls developmental timing and performs as a mechanism thatmediates dauer entry switching Whenanimal loss the function of the Lin-42 it will be get oversensitive todauer form with stressfulcondition Tennessen J M Opperman K J Rougvie A E 2010The project fishing for Protein was to classify unknown genes which were associated withregulation of the developmental timing in C elegans There were three parts the project
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