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Hypothesis Enzymes such as Catalase are large protein molecules that are found in living cells They are used to speed up specific reactions in the cells They are all specific as each enzyme just performs one particular reaction In their globular structure one or more polypeptide chains twist and fold bringing together a small number of amino acids to form the active site or the location on the enzyme where the substrate binds and the reaction takes place Enzyme and substrate fail to join together if their shapes do not match exactly This ensures that the enzyme does not participate in the wrong reaction The enzyme itself is unaffected by the reaction When the products have been released the enzyme is ready to bind with a new substrate Enzymes work to change the rate of a reaction without being absorbed by the reaction The reactant that an enzyme acts on is called the enzymes substrate The product is what the enzymes convert the substrate into Substrate Enzyme Product Catalase is an enzyme found in food such as potato and liver It is used for removing Hydrogen Peroxide from the cells Hydrogen Peroxide is the poisonous by-product of metabolism Catalase speeds up the decomposition of Hydrogen Peroxide which is the substrate into water and oxygen as shown in the equations below Hydrogen peroxide Catalase Water Oxygen H2O2 Catalase H2O O2 It is able to speed up the decomposition of Hydrogen Peroxide because the shape of its active site matches the shape of the Hydrogen Peroxide molecule This type of reaction where a molecule is broken down into smaller pieces is called an anabolic reaction In this experiment Apparatus Conical flask Rubber bung Gas
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