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Coursework - Osmosis Planning Aim To investigate what concentration of sucrose solution is isotonic to potato cell sap Apparatus0M sucrose aq distilled water 2M sucrose aq Potato Chip cutter Knife Boiling tubes Measuring cylinder 25cm3 Vernier callipers to nearest 01mm Balance to nearest 001gram Safety I will ensure safety around the lab by wearing safety goggles laying paper towels removing baggage from walking space push stools under the bench stand up walk carefully and slowly - dont rush and handle equipment and apparatus carefully Preliminary Experiment I did a small experiment before doing the real one so I could find a suitable range to base my investigation round I used 3 chips in 3 boiling tubes each containing 20cm3 of 0M sucrose 05M sucrose and 1M sucrose Moles of Sucrose MOriginal Mass gMass after 3 hours gUpDown in mass 0M802994Up 05M818783Down 1M883720Down As you can see from my preliminary results I should investigate between 0M sucrose and 05M sucrose as a suitable range I predicted that the isotonic concentration lies between 035M and 04M MethodI will cut 5 chips with no skin on them and have them approximately the same length width breadth and mass using the balance and Vernier callipers for extra accuracy then I will dry off the sap completely I will use the measuring cylinder to accurately make 20cm3 01M sucrose 20cm3 02M sucrose 20cm3 03M sucrose 20cm3 04M sucrose and 20cm3 05M sucrose each putting it into a boiling tube I will then place the 5 identical chips in each boiling tube and leave for 3 hours so osmosis can occur fully Scientific Knowledge A potatos cell has a fully permeable cell wall but a selectively permeable cell membrane which allows certain substances to enter and not enter the cell such as water and sucrose in this instance In all things everything moves from high concentration areas to low concentration areas when in water this process is called Osmosis With
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