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Word Count: 1,084
Experiment showing the determination of Creatinine concentration in 24-hour urine samples Aim The aim of the practical is to test the following hypothesis The amount of creatinine found in a persons 24-hour urine sample bears a direct relationship to the body mass of that person METHOD Materials 15 urine samples of unknown concentration numbered 1 5 6 7 ad 8 2Picric Acid 3075M Sodium Hydroxide 4P1000 Gilson Pipette 51cm3 5cm3 and 10cm3 glass pipettes 6Vortex Mixer 7Spectrophotometer 8Prafilm 9Test Tubes Prior to the beginning of the experiment students were give data for the standard curve of creatinine concentration ranging from 005-05mM Students were also told that creatinine concentrations in a 24-hour sample are usually between 5-20mM Therefore the five samples would have to be diluted PROCEDURE Dilution factors of x10 x20 x40 x80 and x10 diluted sample 1 5 6 7 and 8 respectively The x10 dilution was prepared by taking 1cm3 of sample 1 and adding this to 9cm3 of distilled water in a clean test tube this was called the primary procedure The test tube was labeled 1d The x20 dilution factor was achieved by initially carrying out the primary procedure however sample 1 is substituted with sample 5 From this x10 dilution of sample five 5cm3 is removed and added to 5cm3 of water in a clean test tube this gave a x20 dilution the test tube was labeled 5d The x40 dilution was made by initially repeating the primary procedure however sample 1 is substituted with sample 6 From this x10 dilution of sample 6 3cm3 is extracted and added to 1cm3 distilled water in a clean test tube this made the x40 dilution the test tube was labeled 6d First carrying out the primary procedure made the x80 dilution factor however sample 1 was substituted with sample 7 From this x10
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