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Abstract The topic of this research involved the occurrence of genetic transformation in bacteria E Coli More specifically a previously prepared pGLO plasmid--which consisted of the gene to be cloned--was used to transform non-pathogenic bacteria The pGLO plasmid contained a gene for the Green Fluorescent Protein GFP from a bioluminescent jellyfish and a gene for resistance to ampicillin an antibiotic Essentially we wanted to determine the conditions of the bacteria that would glow Our hypothesis was that the transformed solution with no plasmid DNA and ampicillin would produce no bacteria colonies as it wouldnt be able to grow without the gene for ampicillin resistance Also the transformed solution with just LB and ampicillin would produce bacteria colonies but the transformed solution with LBampicillinArabinose would produce glowing bacteria colonies as Arabinose allows the GFP gene to be expressed but in both cases bacteria colonies would be present because of the gene of resistance to the antibiotic ampicillin We essentially made the required transformed solutions--and the controls--swiped them on the agar plate and then observed to see whether or not bacteria colonies grew and whether or not they glowed Our data fully supported our hypothesis We can thus conclude that bacteria can take in foreign DNA through the process of transformation and that this foreign DNA can fundamentally change the bacteria ex making it glow Future research can involve inserting other pieces of DNA into bacteria from different organisms making the bacteria take on various phenotypic characteristics Background Information Genetic transformation is one of the most important processes in biotechnology Essentially genetic transformation involves the process where a cell in this lab a bacterial cell takes up foreign DNA from its surroundings and incorporates it into its own DNA This gene transfer is accomplished with the aid of a plasmid a naturally-occuring small piece of circular DNA in bacteria Bacteria can efficiently adapt to new environments by transferring plasmids with beneficial genes aiding survival
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