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Introduction This experiment involves an extraction of a natural product using the techniques of steam distillation The principle component of oil of cloves is an aromatic compound which is identified by thin layer chromatography TLC Eugenol is widely used in dentistry due to its analgesic antiseptic balsamic qualities It is ideal for curative for pulp hyperemia the soft sensitive substance underneath the enamel and dentine of a tooth and pulp its In dentistry eugenol is also used to cover cavities fill canals etc It also keeps food fresh and kills off bacteria and viruses Eugenol helps keep meat fresh and prevents stomach upset diarrhea and wound infections In addition it might play a part in relieving indigestion controlling diarrhea and healing cuts and bites Eugenol is the main active ingredient in cloves Procedure 1 In a 250 mL round bottom flask place 100g of ground cloves and 80 mL of water Set up for a simple distillation 2 Heat to boiling and collect the distillate until you no longer see droplets of organic liquid coming out of the condenser about 40 minutes 3 Place the distillate in a separatory funnel and extract with three 10 mL portions of Dichloromethane 4 Combine the dichloromethane extracts and dry over Sodium Sulfate 5 Examine the purity of the extract using thin-layer chromatography TLC Spot a small sample of the extract using a capillary spotter about 1 cm from the end of TLC plate 6 Visualize using a UV light and mark the visualized spots with a pencil 7 Record the number of spots and the Rf values from the spots 8 While the plate is developing set up a clean and dry distillation set up and distil off most of the dichloromethane solvent 9 The remaining solution of Eugenol in Dichloromethane can be removed using a disposable pipette and placed into a clean pre-weighted vial 10 The vial can be heated on a steam bath under the
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