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ABSTRACT Proteins are the macromolecules that are responsible for most of the bodily functions By investigating an individual protein one can be able to understand the functions and structure of an organism Before this can be done protein has to be separated from cell components Using the methods of centrifugation and gel electrophoresis not only a protein can be separated from cellular components but also from other proteins An experiment was designed to separate protein contained in liver tissue of a cow based on its molecular weight From the centrifugation soluble protein concentration extracted for the electrophoresis Dilution was prepared of this concentrated extract positive correlation made between the concentration of BSA and measured absorbance This correlation helped to draft a regression line which was useful in finding concentration of protein extract An electrotransfer on PVDF membrane was done after the gel electrophoresis These migrations of proteins based on their molecular weight of three solutions Using the polyacrilamide electrophoresis proteins were separated based on their molecular weight Extraction buffer did not produce protein bands Proteins with huge molecular weight did not travel far from the gel while low molecular weight proteins traveled long distance Overall banding contrast was compared between same sample with different transferred volumes The protein bands of ADH were closely related to some of the bands of liver protein extract ADH which is derived from horse contains some of the protein that are related to the proteins found on cows liver cells Closely ranged Rf value is key to similarities that exists between protein bands based on their molecular weight The results of these experiments were compared with research group with similar interests the results and conclusion drawn were closely correlated Introduction Our body consists of various types of macromolecules which are the fundamental functional unit of life These macromolecules are responsible for most of all of the cell functions They include molecules such as carbohydrates glycogen
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