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THE MICROSCOPE EXPERIMENT 1 BACKGROUND The use of a microscope is to provide a magnified view of objects that are being analysed that are otherwise to small to be seen by the naked eye They can be described according to their illumination and lens arrangement iMicroscopes are able to use either light or electrons as their illumination source which are respectively known as light powered and electron microscopes iiMonocular microscopes have a single eye piece where as binocular microscopes posses two eye pieces position side by side for simultaneous viewing with both eyes iiiA simple microscope consists of one single lens system where as a compound microscope consists of two main lens systems an ocular and objective which are superimposed over each other to provide greater magnification In Biology microscopes can also be described according to some specific purpose such as dissecting microscopes which are commonly referred as dissectors are especially suitable for use while dissecting very small or delicate specimens Microscopes are usually equipped with a series of interchangeable eyepiece lenses oculars each with different individual magnifications Majority of ocular magnification is as followed X4 X5 X6 X7 X8 X10 X12 and X15 On a typical monocular microscope objectives magnification found is as followed X4 SCANNING POWER SP X10 LOW POWER LP X40 HIGH POWER HP To find the overall magnification factor obtained when using any microscope is calculated by the following mathematical formula OCULAR magnification X OBJECTIVE magnification OVERALL magnification The condenser lens is situated below the stage and causes light rays to converge on to the specimen situated on the stage thus illuminating is adequately when magnified by the viewing lens The amount of light passing through the condenser lens can be varied by opening and closing the
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