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Introduction The purpose of this investigation was to test the null hypothesis that there is no relationship between different chemical stimuli and their effect on the heart rate To be able to see the effects of the different stimuli a Daphnia was used or as some people call them a water flea The results for each particular stimulus in this experiment will probably be different some will increase the Daphnias heart rate and some will slow its heart rate This will be due to the different reactions of the chemicals to the body As most everyone probably already knows different chemicals such as caffeine will speed up your heart rate and others as alcohol will slow your heart rate Methods The experiment on the Daphnia was performed as follows The experimenters or group members first obtained and set up a microscope on their lab bench Then they used a Pasteur pipette to obtain a Daphnia from the stock supply on the middle lab bench and placed it in a depression of a depression slide making sure there was enough liquid in the depression to sustain the Daphnia They then placed a cover slip over the top of the depression Next the experimenters placed the slide containing the Daphnia on the microscope stage and used the low power objective to focus on the specimen and locate its heart Making sure they turned off the light source when not making observations they allowed the Daphnia to become equilibrated to its surroundings for two minutes before they determined its resting heart rate The resting heart rate was then recorded in table two of their lab print out They then placed the corner of a KimWipe on one side if the cover slip and with a Past eur pipette they dropped a few drops of water from the stock pond one the other side of the cover slip and allowed the water to
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