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Chromatography A widely used separation tool Purpose Given a sample of unknown ink we were to use chromatography paper to determine the identity of the sample And also we were to separate two dyes of different color using a silica gel column made in lab Procedure Lab manual Experimental Chemistry pg47-50 There were no changes made to this lab Experimental measurements and Data See attached page of results from the chromatography experiment There was no data or measurements for the separation of the dyes using the silica gel column Calculations and Data analysis There were no equations used in this lab it was a primarily visual lab With the chromatography paper experiment we were given unknown A After placing both unknown A and a piece with the five pens on it in each of the unknowns water non-acidic ethyl acetate HCl and NaOH we had nice examples of capillary action We looked at the unknown and whether it separated in comparison to all five ink samples Ink 1Ink 2Ink 3Ink4Ink 5Unknown A Water non-acidic ethyl acetate HCl acid NaOH base From this chart we could see that unknown A had common traits with ink 3 through out each test plus by looking at the actual data attached page the colors separated out and the height of the bleeding column match almost identically I would say that unknown A in ink 3 With the separation of the different color dyes in a silica gel column we had a few problems establishing an initial gel column But after many failed attempts we finally got moving There was nothing more to this part of the experiment than to watch what would make each dye separate and how they would separately flow down through the silica gel We then checked the final color to determine the acidity or basic quality of each dye Conclusion Using the chromatography paper we discovered that unknown A was
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