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Hydrogen peroxide has an acidic pH which will break down into water which has a neutral pH This may effect the enzyme action as many enzymes work faster in a neutral environment the only control over this is the fact that it will happen in each test and to consider its effect at the end of the experiment I will need to use the same potato and cut a fresh chip at each stage of the experiment as the catalase may become effected by exposure to the air This may effect results so I will keep the chips as similar as possible The apparatus should have been washed with distilled water and I will use a different conical flask for each test so that any reactants left in a flask wont affect further tests The procedure will stay the same so that the results can be compared Apparatus list and setup - Potato - Potato borer - Ruler - Scalpel - 6 conical flasks - 20ml syringe to hold enough hydrogen peroxide to cover the chip in the boiling tube - Double holed bung - Retort stand - Clamp - Tongs - Goggles - 50ml glass measuring cylinder to measure gas produced accurately and allow space for the displaced gas As the cylinder is larger than necessary I can take the next reading directly after the first without having to fill the cylinder as much - Bowl of water - Hydrogen peroxide 20vol - Splint - Timer - Delivery tube - Tile diagram Method 1 Set up apparatus including filling glass cylinder with water and placing it upside down in water measure width of potato borer and apply this to surface area formula 2 Read the level the glass measuring cylinder shows 3 Cut a piece of potato 4cm and calculate the
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