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PHM499 Research Project Calcium transport study of SF-9 lepidopteran cells and bull frog sympathetic ganglion cells ABSTRACT The intracellular calcium level and the calcium efflux of the bull-frog sympathetic ganglion cells BSG and the SF-9 lepidopteran ovarian cells were investigated using a calcium-sensitive fluorescence probe fura-2 It was found that the intracellular calcium levels were 582 and 447 nM for the BSG cells and SF-9 cells respectively The calcium effluxes following zero calcium solution were 202 and 133 fmolecm-2s-1 for the BSG cells and SF-9 cells The calcium effluxes following sodium orthovanadate Na2VO4 in zero calcium solution were 600 and 080 fmolecm-2s-1 for the BSG cells and the SF-9 cells The SF-9 cells also lost the ability to extrude intracellular calcium after 2-3 applications of Na2VO4 while the BSG cells showed no apparent lost of calcium extruding abilities for up to 4 applications of Na2VO4 INTRODUCTION Spodoptera frugiperda clone 9 SF-9 cells are a cultured insect cell line derived from the butterfly ovarian tissue SF-9 cells are used by molecular biologists for the studies of gene expression and protein processing Luckow and Summers 1988 However there is not much known about these cells basic biophysiology Since calcium is involved in many cells activities such as acting as a secondary messenger it is important for cells to control their intracellular calcium level This study was aimed toward looking at the some of the basic properties of the SF-9 cells such as resting calcium concentration and rate of calcium extrusion after being calcium level being raised by an ionophore 4-bromo-A23187 The effect of sodium orthovanadate an active transport inhibitor on calcium extrusion was also looked at Microspectrofluorescence techniques and the calcium-sensitive probe fura-2 were used to measure the intracellular calcium concentration of these cells In addition the BSG cells were used to co mpare with the SF-9 cells for the parameters that were studied It was found that the
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