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Introduction Transport processes are based on the movement of matter when a force is exerted upon them 1 Diffusion centrifugation and electrophoresis are all based on forces causing movement or flow of particles Diffusion is the migration of particles based on a concentration gradient Sedimentation and electrophoresis are two transport processed in which the force exerted serves to separate particles Sedimentation depends upon an increase in centrifugal force to cause a flow of matter Electrophoresis is performed with an electrical field to provide the force necessary to cause the migration of particles through a medium The purpose of this study is to use the transport processes of sedimentation and electrophoresis to isolate and examine certain components of prokaryotic and eukaryotic ribosomes Using differential centrifugation 70S Ecoli Mre 600 ribosomes and 80S wheat germ ribosomes will be isolated A protein extract from these ribosomes will be obtained by acetic acidacetone precipitation and analyzed by a SDS-PAGE Sodium dodecylsulfate polyacrylamide gel A CHCl3 extraction method will be employed to isolate the RNA components of the ribosomes which will be analyzed on an agarose gel Standards will be used in both gels and results will be compared to literature data Differential centrifugation works upon the principle that the force on various molecules in a centrifugal field differs depending on mass Fc the centrifugal force on a molecule will equal w2rm1-vpwhere r is the radius m is the mass w is the angular velocity of the centrifuge and 1-vp is the specific density of the molecule This force differs between molecules due to varying masses of molecules There is also a frictional force on the particle that depends on mass The Svedberg constant is defined as m1-vpf a sedimentation constant which varies between molecules The protein extract which was obtained using an acetic acidacetone precipitation was further analyzed using and SDS-PAGE Specifically discontinuous electrophoresis was performed which involves two gels of different pH a
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