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Auxins and their effects on Rooting Introduction Thimann and Koepfli identified growth-promoting and root-forming substances in plants They demonstrated the practical use of IAA in the stimulation of root formation on cuttings Even though there a number of studies indicating that auxins have a promotive effect on rooting there are exceptions which show that it either has no effect on root elongation or is inhibitory at higher concentrations In addition to either exogenous or endogenous auxin effects it has been suggested that the sensitivity responsiveness of the cuttings to auxin may also be very important The proper environmental conditions and physiological status of the stock plant is very important for successful rooting of cuttings Stock plants should have the following optimized water temperature light nutrition and CO2 enrichment Cutting are typically taken early in the day when they are still turgid If they cannot be planted immediately they are misted to reduce transpiration and held at 4-8C until they can be planted However the key point is that once the cutting is taken from the parent plant it is very important to maintain its water status and minimize dry matter loss It is important to take into account that when IAA solutions are kept under nonsterile conditions andor sunlight they will rapidly be destroyed this is not a problem with synthetic auxins The acid form of auxin is only soluble in water at very low concentrations In order to solubilize higher concentrations of auxin in its acid form it must be first dissolved in a few drops of alcohol or ammonium hydroxide prior to slowly adding to water There are several direct benefits of using auxins such as a higher percent of cuttings produce roots root initiation is typically quicker the number and quality of roots per cutting is increased and the uniformity of rooting along the length of cutting in increased There are three methods which have come into widespread practical
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